Craniofacial and Molecular Genetics
Welcome to the Division of Craniofacial and Molecular Genetics, Directed by Dr. Pamela C. Yelick, PhD. Dr. Yelick is a tenured full professor in the Department of Orthodontics, Tufts University School of Dental Medicine, and also holds adjunct appointments in the Cell Molecular and Developmental Biology, Genetics, and Pharmacology Programs in the Sackler School of Graduate Biomedical Sciences, Tufts Medical School, Boston, MA, and in the Department of Biomedical Engineering, School of Arts and Sciences, Tufts University, Medford, MA.
Research in the Yelick Lab focuses on studying the molecular signaling cascades regulating the processes of mineralized tissue – craniofacial, skeletal, tooth - development, homeostasis, disease and regeneration. Craniofacial and skeletal diseases can be quite serious and debilitating. A better understanding of the genetic basis of these diseases, as well as their progression, can lead to new and more effective therapies for affected individuals.
Dr. Yelick holds extensive published expertise in basic research pertaining to craniofacial development and regeneration using the zebrafish and mouse models, and in clinically relevant dental tissue engineering research models, using three-dimensional dental cell-seeded scaffolds implanted into rat and pig models, for eventual applications in dental tissue and whole tooth regeneration. Dr. Yelick has received NIH funding since 1990 and recently received AFIRM 2 R01 and NIH/NIDCR R21 awards.
Dr. Yelick is a reviewer for numerous journals, including: Archives of Oral Biology, Biomaterials, BMC Developmental Biology, Calcified Tissue International, Development, Developmental Biology, Developmental Dynamics, European Journal of Oral Sciences, Experimental Cell Research, FASEB Journal, Oncogene, PLoS, Journal of Biomedical Materials Research, Science, Journal of Clinical Investigation, Journal of Dental Research, Journal of Pathology, Mechanisms of Development, Nature, PNAS, Science and Tissue Engineering.
She was a permanent member of the NIH Musculoskeletal Tissue Engineering Study Section, an ad hoc reviewer for many NIH Study Sections including ODCS, SBDD and Special Emphasis NIH Study Section Panels, and also serves as a reviewer for the California Institute of Regenerative Medicine (CIRM) and NSF. She recently served a 3 year elected term as Treasurer of the American Association for Dental Research (AADR). Dr. Yelick also serves on the Scientific Advisory Boards for Tissue Engineering and Regenerative Medicine International Society-Americas (TERMIS-AM) and TERMIS-European Union (TERMIS-EU), and TERMIS-Asia Pacific (TERMIS-AP).
Dr. Yelick is an internationally recognized leader in dental tissue engineering and craniofacial development, with over 80 peer-reviewed basic research publications, more than a dozen reviews, and over 200 abstracts since the year 2000. She has received national and international acclaim for her research on dental tissue and whole tooth tissue engineering, and has participated in over 200 Invited Speaker Lectureships.
She has extensive teaching experience, and to date has mentored 23 Postdoctoral Fellows, 7 Graduate Students, 33 Research Technicians, 32 Dental Students, and numerous High School and undergraduate students. Her laboratory regularly hosts International Visiting Scientists to pursue cutting-edge research.
Members of the Division of Craniofacial and Molecular Genetics
Ms. Quan Diep
Dr. Weibo Zhang, DMD, PhD
Dr. Elizabeth Smith, PhD
Sara Rudolph, BA
Dr. Mohammed Barashi, DMD, MA, Orthodontics 2018
Alex Cintolo, BA
Alen Blagajcevic, D’21
Rebecca Fisher, D’21
Alicia Kim, D’21
Formers Yelick Lab Members:
2014-2018 Melissa LaBonty, PhD 2018 Sackler School of Biomedical Sciences
2015-2018 Nelson Monteiro, PhD, Postdoctoral Fellow
Current Yelick Research Support
R01, NIH/NIDCR/NIBIB (PI, Yelick)
“Bioengineered composite alveolar bone-tooth constructs for tooth regeneration.”
Tooth loss with associated alveolar jaw bone resorption is a wide-spread affliction currently affecting more than 15M individuals in the US alone. In addition, craniofacial birth defects including cleft lip/palate occur in 1/700 live births, and often require both alveolar jaw bone and tooth replacement therapies, which need to be performed sequentially over time in order to achieve proper form and function in growing children. The need for cancer resections and the widespread occurrence of accidental and battlefield injuries affecting the craniofacial complex, all require significant surgical intervention to restore the proper form and function of the jaws and dentition. As such, the need for effective Tissue Engineering and Regenerative Medicine (TERM) therapies for craniofacial jaw and tooth regeneration are a significant health concern. To date, reliable methods to repair human jaws and teeth are quite limited, and exhibit significant deficiencies including extensive healing times, the need for multiple sequential surgeries, limited and often inadequate cell and bone tissue sources, donor site morbidities associated with autologous bone harvesting, and tooth regeneration therapies that are largely limited to ankylosed dental implants, which are not suitable for many individuals who have undergone extensive mandibular and maxillary bone resorption, and which do not exhibit any of the properties of natural teeth. As such, identifying reliable methods to regenerate alveolar bone and teeth is a significant health priority for treatment for a variety of genetic, age-associated and injury based craniofacial defects.
- Specific Aim 1. Validate decellularized natural tooth bud ECM (tbECM) scaffolds for full sized tooth and alveolar bone regeneration. Here we will build upon our previously published reports to validate reliable methods to use decellularized tooth bud scaffolds for coordinated alveolar bone and tooth regeneration.
- Specific Aim 2. Validate bioengineered 3D hydrogel tooth bud constructs for tooth and jaw bone regeneration. Here we will build upon our published reports to validate GelMA and silk hydrogel scaffolds for coordinated alveolar bone and tooth regeneration.
2017-2019 R21, NIH/NIAMS, (PI, Yelick)
“Novel zebrafish models for human Fibrodysplasia Ossificans Progressiva.”
The proposed studies will provide insight into the molecular genetic basis of the debilitating human disease Fibrodysplasia Ossificans Progressiva (FOP), in which an individual’s soft connective tissues progressively ossify over time eventually resulting in untimely death. In addition, heterotopic ossification (HO) of soft tissues is a common health issue associated with aging, and in calcific arterial valve disease (CAVD), osteoarthritis, and atherosclerosis. Although recent studies have revealed that activating mutations in human ACVR1/ALK2 are causative in FOP, a detailed knowledge and understanding of the tissue and cell types contributing to the disease, and of the molecular signaling pathways involved in FOP disease progression, remain to be fully elucidated. The long-term goal of our research is to develop new drug therapies to prevent and/or treat human FOP and HO, by establishing and characterizing new animal models for, and gene targets associated with, these diseases. This two year R21 grant will validate novel zebrafish models for human FOP, and to use them to identify novel molecular targets to treat and/or halt the progression of FOP.
- Specific Aim 1. Create and validate FOP zebrafish.
Hypothesis: Transgenic zebrafish mimic the human FOP heterotopic ossification (HO) phenotype.
- Specific Aim 2. Define cell type-specific contributions to HO in FOP zebrafish.
Hypothesis: Distinct cell types contribute to the progression of HO in human FOP.
2017-2018 FOP Competitive Research Grant, Co-PI with Dr. Edward Hsiao, UCSF PI
International Fibrodysplasia Ossificans Progressiva Association (IFOPA)
“Interrogating new therapeutic targets for human FOP using Zebrafish.”
The major goals of this study are to devise new and effective methods to treat patients with FOP. The objective of the proposed studies is to use our recently validated zebrafish FOP model to interrogate single nucleotide polymorphisms (SNPs) identified in the DNA of FOP patients exhibiting an apparent resistance to FOP progression. The objective of the studies proposed here is to test how inhibition of each of these genes affects FOP disease progression in two aims:
- Specific Aim 1. Test how SNPs associated with FOP mild phenotypes interact with Acvr1-R206H in zebrafish.
- Specific Aim 2. Identify and sequence a validation cohort of additional mild FOP patients.
2015-2017 Osteo Science Foundation, (PI, Yelick) “Bioengineered alveolar bone and tooth constructs.”
Here we investigate new methods to regenerate jaw bone, and jaw bone and tooth defects, using novel biphasic tyrosine-based scaffolds seeded with human dental pulp derived stem cells (hDSCs). hDSCs are a superior cell source for jaw bone formation, as compared to bone marrow stromal derived stem cells (hBMSCs), due to the fact that hDSCs naturally give rise to craniofacial jaw bone and teeth. We also believe that tyrosine-derived polycarbonate based scaffolds (TyrPCs) are a superior scaffold choice for craniofacial jaw and tooth repair, due to their superb properties including: biocompatibility; controlled biodegradability; and high macro- and micro-porosity that allows for high cellularity throughout the construct (Kim, Magno et al. 2012).
- Specific Aim 1. Validate hDSC seeded E1001-1K scaffolds for in vivo alveolar bone regeneration.
- Specific Aim 2. Validate hDSC seeded E1001-1K scaffolds for in vivo alveolar bone and tooth regeneration.
2013-2018 AFIRM 2 Award, AFIRM-WRC, (PI, Yelick)
Co-PI Dr. Joachim Kohn
“Engineering alveolar bone and tooth constructs.”
The subject of our study is to fabricate biphasic, biodegradable scaffolds to facilitate the repair and regeneration of functional alveolar jaw bone, and alveolar jaw bone and teeth. Craniofacial jaw and dental defects represent a serious health issue, since proper function and aesthetics of the craniofacial complex is required for eating, communication, and psychosocial interactions. Since craniofacial injuries account for 80% of all battlefield injuries, these studies address an important unmet medical need for the military. The purpose of our study is to devise improved methods for repair of craniofacial jaw and tooth defects in a timely and highly functional manner. The scope of our study is to devise improved methods for efficient and effective repair and regeneration of craniofacial jaw and tooth defects incurred in the line of duty.
- Fabricate scaffolds for optimized alveolar bone formation
- Demonstrate hDSC viability on scaffolds using in vitro methods.
- Demonstrate dental hDSC viability on scaffolds using in vivo methods.
- Demonstrate alveolar bone formation on in vivo hDSC seeded scaffolds.
Original Reports (in chronological order):
- Hecht, N.B., Kleene, K.C., Yelick, P.C., Johnson, P.A, Pratvtcheva, D.D., Ruddle, F.H. (1986) Mapping of haploid expressed genes: genes for both mouse protamines are located on chromosome 16. Somatic Cell and Molecular Genetics 12(2):203-208.
- Hecht, N.B., Bower, P.A., Waters, S.H., Yelick, P.C., Distel, R.J. (1986) Evidence for haploid expression of mouse testicular genes. Experimental Cell Research 164:183-190.
- Yelick, P.C., Balhorn, R., Johnson, P.A., Corzett, M., Mazrimas, J.A., Kleene, K.C., Hecht, N.B. (1987) Mouse protamine 2 is synthesized as a precursor whereas mouse protamine 1 is not. Molecular and Cellular Biology 7:2173-2179.
- Bower, P.A., Yelick, P.C., Hecht, N.B. Both P1 and P2 protamine genes are expressed in mouse, hamster and rat. (1987) Biology of Reproduction 37:479-488.
- Johnson, P.A, Peschon, J.J., Yelick, P.C., Palmiter, R.D., Hecht, N.B. (1988) Sequence homologies in the mouse protamine 1 and 2 genes. Biochimica et Biophysica Acta 950:45-53.
- Johnson, P.A, Yelick, P.C., Leim, H., Hecht, N.B. (1988) Differential distribution of the P1 and P2 protamine genes sequences in Eutherian and marsupial mammals and a monotreme. Gamete Research 19:169-175.
- Kleene, K.C., Borzorgzadeh, A., Flynn, J.F., Yelick, P.C., Hecht, N.B. (1988) Nucleotide sequence of a cDNA clone encoding mouse transition protein 1. Biochimica et Biophysica Acta 950:215-220.
- Hecht, N.B., Distel, R.J., Yelick, P.C., Tanhauser, S.M., Driscoll, C.E., Goldberg, E., Tung, K.S. (1988) Localization of a highly divergent mammalian testicular alpha-tubulin that is not detectable in brain. Molecular and Cellular Biology 8:996-1000.
- Mali, P, Sandberg, M, Vuorio, E, Yelick, P.C., Hecht, N.B., Parvinen, M. (1988) Localization of protamine 1 mRNA in different stages of the cycle of the rat seminiferous epithelium. Journal of Cell Biology 107:407-412.
- Yelick, P.C., Kwon, Y.K., Flynn, J.S., Borzogzadeh, A., Kleene, K.C. Hecht, N.B. (1989) Mouse transition protein 1 is transitionally regulated during the postmeiotic stages of spermatogenesis. Molecular Reproduction and Development 1:193-200.
- Reeves, R.H., Gearhart, J.C., Hecht, N.B., Yelick, P.C., Johnson, P.A., O'Brien, S.J. (1989) Mapping of PRM1 to Human Chromosome 16 and Tight Linkage of Prm-1 and Prm-2 on Mouse Chromosome 16. Journal of Heredity 80:442-446.
- Yelick, P.C., Kozak, C., Kwon, Y.K., Seldin, M.F., Hecht, N.B. (1991) The mouse transition protein 1 gene contains a B1 repetitive element and is located on chromosome 1. Genomics 11:687-694.
- Li, Y.P., Alexander, M., Wucherpfennig, A.L., Yelick, P.C., Chen, W., Stashenko, P. (1995) Cloning and complete coding sequence of a novel human cathepsin expressed in giant cells of osteoclastomas. J Bone Min Res 10(8):1197-1202.
- Yelick, P.C., Driever, W., Neuhauss, S., Stashenko, P. (1996) Craniofacial cartilage development in zebrafish. Ann. NY Acad. Sci. 785:360-361.
- Yelick, P.C., Abduljabbar, T.S., Stashenko, P. (1998) zALK-8, a novel type I Serine/Threonine Kinase Receptor, is expressed throughout early zebrafish development. Developmental Dynamics 211:352-361. (Cover photograph).
- Postlethwait, J.H., Yan, Y.L., Gates, M.A., Horne, S., Amores, A., Brownlie, A., Donovan, A., Egan, E.S., Force, A., Gong, Z., Goutel, C., Fritz, A., Kelsh, R., Knapik, E., Liao, E., Paw, B., Ransom, D., Singer, A., Thomson, M., Abduljabbar, T.S., Yelick, P.C., Beier, D., Joly J.S., Larhammar, D., Rosa, F., Westerfield, M., Zon, L.I., Johnson, S.L., Talbot, W.S. (1998) Vertebrate genome evolution and the zebrafish gene map. Nature Genetics 18:345-349.
- Payne, T.L., Postlethwait, J.H. and Yelick, P.C. (2001) Functional characterization and genetic mapping of alk8. Mechanisms of Development 100:275-289.
- Babb, S.G., Barnett, J., Doedens, A.L., Cobb, N., Liu, Q., Sorkin, B.C., Yelick, P.C., Raymond, P.A. and W.J., Marrs, J.A. (2001) Zebrafish E-cadherin: expression during early embryogenesis and regulation during brain development. Developmental Dynamics 221(2):231-7.
- Payne, T.L., Skobe, Z., and Yelick, P.C. (2001) Regulation of Tooth Development by the Type I TGFb Family Member Receptor Alk8. Journal of Dental Research 80 (11):1968-1973.
- de Caestecker, M.P., Bottomley, M., Bhattacharyya, S., Payne, T.L., Roberts, A.B., Yelick, P.C.. (2002) The novel type I serine-threonine kinase receptor Alk8 binds TGF-beta in the presence of TGF-b RII. Biochem Biophys Res Commun 293(5):1556-65.
- Bang, P., Yelick, P.C., Malicki, J., and Sewell, W. (2002) High-throughput behavioral screening method for detecting auditory response defects in zebrafish. J. Neuroscience Methods, 118(2): 177-187.
- Young, C.S., Terada, S., Vacanti, J.P., Honda, M., Bartlett, J.D., and Yelick, P.C. (2002) Tissue engineering of complex tooth structures on biodegradable polymer scaffolds. Journal of Dental Research, 81(10):695-700.
- Young, C.S., Terada, S., Vacanti, J.P., Masaki, H., Bartlett, J.D., and Yelick, P.C. (2003) The Regenerated Tooth: Tissue engineering of complex tooth structures on biodegradable polymer scaffolds. Journal of Dental Technology, April/May, pp 21.
- Payne-Ferreira T.L., Yelick P.C. (2003) Alk8 is required for neural crest cell formation and development of pharyngeal arch cartilages. Developmental Dynamics (Cover Photograph) 228:683-696.
- Perrino, M.A. and Yelick, P.C. (2004) Immunolocalization of Alk8 during replacement tooth formation in zebrafish. Cells, Tissues and Organs 176:17-27.
- Payne-Ferreira, T., Tong, K. W., and Yelick, P.C. (2004) Maternal alk8 promoter fragment directs expression in early oocytes. Zebrafish, 1(1):27-39.
- Duailibi, M. T., Duailibi, S.E., Young, C.S., Bartlett, J.D., Vacanti, J.P. and Yelick, P. C. (2004) Bioengineered teeth from cultured rat tooth bud cells. Journal of Dental Research 83(7):523-8.
- Young, C.S., Kim, S.-W., Qin, C., Baba, O., Butler, W.T., Taylor, R.R., Bartlett, J.D., Vacanti, J.P. and Yelick, P.C. (2005) Developmental analysis and computer modeling of bioengineered teeth. Archives in Oral Biology, 50(2):259-265.
- Albertson, R.C. and Yelick, P.C. (2005) Roles for Fgf8 signaling in left-right patterning of the visceral organs and craniofacial skeleton. Developmental Biology 283(2):310-21. [Cover Photo]
- Albertson, R. C., Payne, T. L., and Postlethwait, J., and Yelick, P.C. (2005) Zebrafish acvr2a and acvr2b exhibit distinct roles in craniofacial development. Developmental Dynamics 233(4):1405-18.
- Young, C. S., Abukawa, H., Asrican, R., Ravens, M.S., Troulis, M.J., Kaban, L.B., Vacanti, J.P., and Yelick, P.C. (2005) Tissue Engineered Hybrid Tooth and Bone. Tissue Engineering 11(9-10):1599-610.
- Albertson, R.C., Streelman, J. T., Kocher, T., Yelick, P.C. (2005) Integration and evolution of the cichlid mandible: the molecular basis of alternate feeding strategies" Proc Natl Acad Sci U S A 102(45):16287-92. Epub 2005 Oct 26, Cover Photo
- Adams, D.S, Robinson, K.R, Fukumoto, T., Yuan, S., Albertson, R. C., Yelick, P.C, Kuo, L., McSweeney, M., and Levin, M. (2006) Early H+-V-ATPase-dependent proton flux is necessary for consistent left-right patterning of non-mammalian vertebrates. Development 133(9):1657-71. Epub 2006 Mar 22.
- Fang, P.K., Solomon, K.R., Zhuang, L., Qi, M., McKee, M., Freeman, M.R., and Yelick, P.C. (2006) Caveolin-1alpha and -1beta perform non-redundant roles in early vertebrate development. American Journal of Pathology 169(6):2209-2222. [Cover Photo] Also see Related Commentary, Frank, P.G. and Lisanti, M.P. Zebrafish as a Novel Model System to Study the Function of Caveolae and Caveolin-1 in Organismal Biology Am. J. Pathology, 169(6):1910 - 1912.
- Duailibi, S.E., Duailibi, M.T., Vacanti, J.P., and Yelick, P.C. (2006) Prospects for tooth regeneration. Periodontology 2000 41:177-87. Dent Clin North Am 50:191-203.
- Yelick, P.C., Vacanti, J.P. (2006) Bioengineered teeth from tooth bud cells. Dent Clin North Am 50(2):191-203.
- Albertson, R.C., Yelick, P.C. (2007) Fgf8 haploinsufficiency results in distinct craniofacial defects in adult zebrafish. Dev. Biol 306(2):505-15 (March 24 Epub ahead of print).
- Cinar, B., Fang, P.K, Lutchman, M., Di Vizio, D., Adam, R.M., Pavlova, N., Rubin, M., N., Yelick, P.C., and Freeman, M.R. (2007) The pro-apoptotic kinase Mst1 and its caspase cleavage products are direct inhibitors of Akt1. EMBO Journal 26(21):4523-34. Epub 2007 Oct 11.
- Xu, W.P., Zhang, W., Asrican, R., Kim, H.J., Kaplan, D.L., Yelick, P.C. (2008) Accurately shaped tooth bud cell-derived mineralized tissue formation on silk scaffolds. Tissue Eng Part A. 14(4):549-57.
- Jezewski, P.A., Fang, P.-K., Payne-Ferreira, T.L., and Yelick, P.C. (2008) Zebrafish wnt9b synteny and expression during first and second arch, heart, and pectoral fin bud morphogenesis. Zebrafish 5(3):169-77.
- Duailibi, S.E., Duailibi, M.T., Zhang, W., Asrican, R., Vacanti, J. P. and Yelick, P.C. (2008) Bioengineered dental tissues grown in the rat jaw. Journal of Dental Research 87(8):745-50.
- Zhang, W., Abukawa, H., Troulis, M., Kaban, L., Vacanti, J.P., Yelick, P.C. (2008) Tissue engineered hybrid tooth-bone constructs. Tissue Engineering Methods 47(2):122-8 Epub 2008 Oct 7.
- Abukawa, H., Zhang, W., Young, C.S., Asrican, R., Vacanti, J.P., Kaban, L.B., Troulis, M.J., Yelick, P.C. (2009) Reconstructing mandibular defects using autologous tissue-engineered tooth and bone constructs. Journal of Oral and Maxillofacial Surgery 67(2):335-47.
- Jezewski, P., Fang, P-K., Payne-Ferreira, T.L. and Yelick P.C. (2009) Alternative splicing, phylogenetic analysis, and craniofacial expression of zebrafish tbx22. Dev. Dyn. 238(6):1605-12.
- Albertson, R.C., Yan, Y.L., Titus, T.A., Pisano, E., Vacchi, M., Yelick, P.C., Detrich, H.W. 3rd, Postlethwait, J.H. (2010) Molecular pedomorphism underlies craniofacial skeletal evolution in Antarctic notothenioid fishes. BMC Evolutionary Biology 10(1):4.
- Connolly, M. and Yelick, P.C. (2010) High throughput methods for visualizing the teleost skeleton: capturing autofluorescence of alizarin red. The Journal of Applied Ichthyology 26:274-277.
- Yelick, P.C. and Connolly, M. (2010) A forward genetic screen for genes regulating mineralized tooth and bone formation in zebrafish. The Journal of Applied Ichthyology 26:192-195.
- Lin, Y., Gallucci, G.O., Busser, D., Bosshardt, D., Belser, U.C., and Yelick, P.C. (2011) Bioengineered periodontal tissue formed on titanium implants. Journal of Dental Research 90(2):251-6. (Dec 13, Epub ahead of print).
- Zhang, W., Ahluwalia, I. P., Yelick, P.C. (2010) Three dimensional dental epithelial-mesenchymal constructs of predetermined size and shape for tooth regeneration. Biomaterials 31(31):7995-8003. Epub 2010 Aug 1.
- Zhang, W., and Yelick, P.C. (2010) Vital pulp therapy-current progress of dental pulp regeneration and revascularization. International Journal Dent. 2010:856087. Epub 2010 Apr 28.
- Zhang, W., Ahluwalia, I.P., Literman, R., Kaplan, D.L., and Yelick, P.C. (2011) Human dental pulp progenitor cell behavior on aqueous and hexafluoroisopropanol (HFIP) based silk scaffolds. Journal of Biomedical Materials Research A. 97(4):414-22.
- Andreeva, V., Connolly, M.H., Stewart-Swift, C., Fraher, D., Burt, J., Cardarelli, J., Yelick, P.C. (2011) Identification of adult mineralized tissue zebrafish mutants. Genesis 49:360-6 (Epub ahead of print. Jan 10, 2011).
- Duailibi MT, Duailibi SE, Duailibi Neto EF, Negreiros RM, Jorge WA, Ferreira LM, Vacanti JP, Yelick PC. (2011) Tooth tissue engineering: optimal dental stem cell harvest based on tooth development. Artif Organs. 35(7):E19-35. 2011 Jun 27. [Epub ahead of print]
- Parsons KJ, Andreeva V, James Cooper W, Yelick PC, Craig Albertson R. (2011) Morphogenesis of the zebrafish jaw: development beyond the embryo. Methods Cell Biol. 101:225-48.
- Duailibi, M.T., Kulikowski, L.D., Duailibi, S.E., Lipay, M.V., Melaragno, M.I., Ferreira, L. M., Vacanti, J. P., and Yelick, P.C. (2011) Cytogenetic instability of dental pulp stem cell lines. J Mol Histology 43(1):89-94. Nov 23, 2011 [Epub ahead of print]
- Andreeva, V., Cardarelli, J., Yelick, P.C. (2012) Rb1 mRNA is expressed in both dental epithelial and dental mesenchymal tissues in developing mouse teeth. Gene Expression Patterns 2012 Jan 25 [Epub ahead of print].
- Zhang, W., Vasquez, B., Spear, D., Hinds, P., Andreeva, V., Yelick, P.C. (2012) Discrete phosphorylated Retinoblastoma protein isoform expression in mouse tooth development. J Mol Histol 2012 Apr 4 [Epub ahead of print].
- Traphagen, S. B., Fourligas, N., Georgakoudi, I., Kaplan, D. L., Yelick, P.C. (2012) Characterization of Natural and Decellularized Porcine Tooth Bud Matrices. Biomaterials 2012 Jul;33(21):5287-96. Epub 2012 Apr 30.
- Ohira, T., Azimi, N., Spear, D., Yelick, P.C. (2012) Chemerin-ChemR23 Signaling in Tooth Development. Journal of Dental Research 2012 Dec 91(12):1147-53 Epub 2012 Oct 9.
- Duailibi, M. T., Duailibi, S. E., W. A., Ferreira, L., M., Salmazi, K.I., Salvadori, M. C., de Sa Teixeira, F., Pasquarelli, A., Vacanti, J. P., Yelick, P. C. (2013) Tooth tissue engineering: the influence of hydrophilic surface on nanocrystalline diamond films for human dental stem cells. Tissue Eng Part A. 2013 Dec, 19(23-24):2537-43.
- Van Manen, E.H., Zhang, W., Walboomers, X.F., Yang, R., Ji, W., Yu, Nl, Spear, D.J., Jansen, J.A., Yelick, P.C. (2014) The influence of electrospun fibre scaffold orientation and nano-hydroxyapatite content on the development of tooth bud stem cells in vitro. Odontology 2014 Sept 26. [Epub ahead of print].
- Zhao, C., Andreeva, V., Gibert, Y., Lattanzi, V., Prabhudesai, S., Zhou, Y., Zon, L., McCann, K, Baserga, S. and Yelick, P.C. (2014) Tissue specific roles for the ribosome biogenesis factor Wdr43 in zebrafish development. PLoS Genetics Jan 30; 10(1).
- Zhang, W., Vazquez, B., Yelick, P.C. (2014) Bioengineered post-natal recombinant tooth bud models. Journal of Tissue Engineering and Regenerative Medicine Nov 25. PMID:25424341 [Epub ahead of print].
- Smith, E., Khademhosseini, A. and Yelick, P.C. (2014) Optimization of a biomimetic model for tooth regeneration. Bioengineering Conference (NEBEC), 2014 40th Annual Northeast, IEEE
- Yelick, P.C. and Trainor, P.A. (2015) Ribosomopathies: Global process, tissue specific defects. Commissioned to Rare Diseases. 3(1) January 2015 (ePub ahead of print). Apr 1;3(1):e1025185. doi: 10.1080/21675511.2015.1025185. PMID: 26442198
- Pisciolaro R.L., Duailibi M.T., Novo N.F., Juliano Y, Pallos D, Yelick PC, Vacanti JP, Ferreira LM, Duailibi SE. (2015) Tooth Tissue Engineering: The Importance of Blood Products as a Supplement in Tissue Culture Medium for Human Pulp Dental Stem Cells. Tissue Eng Part A. 2015 Nov;21(21-22):2639-48. doi: 10.1089/ten.TEA.2014.0617. PMID: 26414682
- Adams D.S., Uzel S.G., Akagi J, Wlodkowic D, Andreeva V, Yelick PC, Devitt-Lee A, Pare JF, Levin M. (2016) Bioelectric signaling via potassium channels: a mechanism for craniofacial dysmorphogenesis in KCNJ2-associated Andersen-Tawil Syndrome. J Physiol. 2016 Feb 10. doi: 10.1113/JP271930. [Epub ahead of print] PMID:26864374
- Song, Z., Zhang, X., Jia, S., Yelick, P.C., Zhao, C. (2016) “Zebrafish as models for human ciliopathies.” Journal of Genetics and Genomics J Genet Genomics. Mar 20;43(3):107-20. doi: 10.1016/j.jgg.2016.02.001. Epub 2016 Feb 12. Review.
- Monteiro, N. and Yelick, P.C. (2016) Review. Advances and perspectives in tooth tissue engineering. (In Press). J Tissue Eng Regen Med. 2016 May 6. doi: 10.1002/term.2134. [Epub ahead of print] Review. PMID: 27151766
- Sondalle SB, Baserga SJ, Yelick PC. (2016) The Contributions of the Ribosome Biogenesis Protein Utp5/WDR43 to Craniofacial Development. J Dent Res. 2016 May 24. pii: 0022034516651077. [Epub ahead of print] PMID:27221611
- Smith, E., Zhang, W., Schiele, N. R., Khademhosseini, A., Kuo, C. K., Yelick, P.C. (2016). Developing a biomimetic tooth bud model. Journal of Tissue Engineering and Regenerative Medicine (JTERM) Jan 8. doi: 10.1002/term.2246. [Epub ahead of print] PMID:28066993
- Zhang, W., Zhang, Z., Chen, S., Macri, L., Kohn, J., Yelick, P.C. (2016) Mandibular jaw bone regeneration using human dental cell-seeded tyrosine-derived polycarbonate scaffolds. Tissue Engineering Journal, Part A Jul;22(13-14):985-93. doi: 10.1089/ten.TEA.2016.0166. PMID: 27369635
- Monteiro, N., Smith, E., Angstadt, S., Zhang, W., Khademhosseini, A., Yelick, P.C. (2016). Dental cell sheet biomimetic tooth bud model. Biomaterials Nov;106:167-79. doi: 10.1016/j.biomaterials.2016.08.024. PMID:27565550
- Khayat, A., Monteiro, N., Smith, E., Pagni, S., Khademhosseini, A., Yelick, P.C. (2016) GelMA encapsulated human dental pulp stem cells for pulp regeneration. Journal of Dental Research Feb;96(2):192-199. doi: 10.1177/0022034516682005. Epub 2016 Dec 15. PMID:28106508
- Zhang, W., Vazquez, B. and Yelick, P.C. (2017) Decellularized tooth buds for tooth regeneration. Journal of Dental Research, Jan 1:22034516689082. doi: 10.1177/0022034516689082. [Epub ahead of print] PMID:28118552
- Zhang, W., Yelick, P.C. (2017) Craniofacial Tissue Engineering. Cold Spring Harb Perspect Med. 2017 Mar 27. pii: a025775. doi: 10.1101/cshperspect.a025775. [Epub ahead of print] PMID:28348178
- LaBonty, M., Nicholas N. Pray and Yelick, P.C. (2017) Zebrafish models for human Fibrodysplasia Ossificans Progressiva. Zebrafish Apr 10. doi: 10.1089/zeb.2016.1398. [Epub ahead of print] PMID: 28394244
- Cai, X., Ten Hoopen, S., Zhang, W., Yi, C., Yang, W., Jansen, J.A., Walboomers, F., Yelick, P.C. (2017) Influence of highly porous electrospun PLGA/PCL/nHA fibrous scaffolds on the differentiation of tooth bud cells in vitro. J Biomed Mater Res A., May 24. doi: 10.1002/jbm.a.36120. [Epub ahead of print]
- Labonty, M. and Yelick, P.C. (2017) Animal Models of Fibrodysplasia Ossificans Progressiva. Developmental Dynamics, Invited Review Feb;247(2):279-288. doi: 10.1002/dvdy.24606. Epub 2017 Dec 1. PMID:29139166
- Belus, M., Josey, M., Rogers, M., Elzubier, A., Rose, S., Andreeva, V., Yelick, P.C., Bates, E.A. (2018) Kir2.1 is important for BMP signaling to correctly pattern the mammalian face. Developmental Biology, Dev Biol. 2018 Mar 20. pii: S0012-1606(17)30829-1. doi: 10.1016/j.ydbio.2018.02.012. [Epub ahead of print] PMID: 29571612
- Smith, E., Angstadt, S., Monteiro, N., Zhang, W., Khademhosseini, A. and Yelick, P.C. (2018) Bioengineered tooth buds exhibit features of natural tooth buds. Journal of Dental Research (In Review)
- LaBonty, M. and Yelick, P.C. (2018) Zebrafish models of Fibrodysplasia Ossificans Progressiva. Bone Morphogenetic Proteins Methods and Protocols, Methods in Molecular Biology, Chapter (In Review).
- LaBonty, M., Pray, N. and Yelick, P.C. (2018) Injury of adult zebrafish expressing Acvr1lQ204D does not result in heterotopic ossification. Zebrafish Journal (In Review).
- Ascoli, P., Bandiera, N., Kataria, S., Katz, B., Slocum, J., Wentworth, M., Monteiro, N., Smith, E., Yelick, P.C. (2018) A device for the mechanical stimulation of cell-seeded scaffolds for dental engineering. Precision Engineering (In Review)
- Zhang, W., Wu, X., Pashuck, T., Sha Mohammadi, M., Kohn, J. and Yelick, P.C. (2018) Differentiation of human dental pulp cell seeded biphasic tyrosine-derived polycarbonate scaffold constructs. Tissue Engineering Journal Part A (In Preparation).
- Monteiro, N., Smith, E., Zhang, W. and Yelick, P.C. (2018) GelMA hydrogel dental cell sheet constructs for whole tooth tissue engineering. (In Preparation).
- Khayat, A., Monteiro, N. and Yelick, P.C. (2017) Recent advances in pulpal regeneration. Journal of Endodontics (In Preparation)
- Zhang, W. and Yelick, P.C. (2017) Advanced in vivo models for tooth regeneration. (In Preparation).
- Andreeva, V., Zhang, W., Vazquez, B., Hinds, P., Yelick, P.C. (2017) Roles for Rb1 in tooth development. Journal of Dental Research (In Revision).
- Alharthi, K., LaBonty, M., Yelick, P.C. (2017) Roles for ddx10 in craniofacial development. Zebrafish Journal (In Preparation).
- Barashi, M., Monteiro, N., Yelick, P.C. (2017) Biomimetic natural tooth ECM-enhanced silk hydrogel scaffolds for tooth regeneration. (In Preparation).
- Zhang, W., Bassett, J., Truong, T., D’Amore, P.A., and Yelick, P.C. (2017) Tooth Development in VEGF Isoform Transgenic Mice. (In Preparation).
- Albertson, R.C., Connolly, M., Huysseune, A., Yelick, P.C. (2017) The zebrafish laf/alk8 mutant exhibits primary and replacement tooth phenotypes. (In Preparation)
- Lee, Y. and Yelick, P.C. (2017) Characterization of the continuously erupting zebrafish dental stem cell niche. (In Preparation).
Books and Other Monographs:
Yelick, Pamela C., “Characterization of Two Genes Involved in the Nuclear Condensation Events of Mouse Spermatogenesis” [Ph.D. Dissertation] November 1989, Tufts University, Medford, MA.
Invited Review Articles (In reverse chronological order)
- LaBonty, M. and Yelick, P.C. (2017) Animal models for FOP. Developmental Dynamics (In Review).
- Zhang, W., and Yelick, P.C. (2017) “Craniofacial Tissue Regeneration.” Cold Spring Harbor Press (In Press).
- Smith, E. and Yelick, P.C. (2016) “Current Progress in Whole Tooth Tissue Engineering.” Current Oral Health Reports Review (In Press)
- Song, Z., Zhang, X., Jia, S., Yelick, P.C., Zhao, C. (2016) “Zebrafish as models for human ciliopathies.” Journal of Genetics and Genomics (In Press)
- Monteiro, N. and Yelick, P.C. (2015) Review. “Advances and Perspectives in Tooth Tissue Engineering.” Journal of Tissue Engineering and Regenerative Medicine (In Press). Wiley
- Zhang, W. and Yelick, P.C. (2013) Chapter 30, Tooth development and regeneration. Biomaterials and Regenerative Medicine. Published by Cambridge University Press.
- Zhang, W. and Yelick, P.C. (2012) Chapter on Scaffolds for Dental Tissue Engineering, “Handbook of Biomimetics and Bioinspiration”, published by World Scientific Publishing.
- Zhang, W. and Yelick, P.C. (2012) Book Chapter, “Nanomaterials for Dental and Craniofacial Tissue Engineering”, Nanomaterials in Tissue Engineering: Characterization, Fabrication and Applications, Woodhead Publishing Ltd.
- Zhang, W. and Yelick, P. C. (2012) CRC’s Biomedical Engineering Handbook, 4th Edition.
- Albertson, R.C., Andreeva, V., and Yelick, P.C. (2011). “Morphogenesis of the jaw – beyond the embryo.” Methods in Cell Biology.
- Zhang, W., and Yelick, P.C. (2010) "Mineralized Tissues in Oral and Craniofacial Science: Biological Principles and Clinical Correlates”, Dental Engineering: Tooth regeneration. (In Press).
- Zhang, W., and Yelick, P.C. (2010) “Vital pulp therapy – progress of dental pulp regeneration and revascularization.” International Journal of Dentistry 2010:856087. Epub 2010 April 28.
- Yen, A., and Yelick, P.C. (2011) “Dental Tissue Regeneration - a mini-review.” Gerontology, Regenerative and Technological. 57(1):85-94. 2010 May 6, Epub ahead of print.
- Traphagen, S. and Yelick, P.C. (2009) “Reclaiming a natural beauty: whole organ engineering with natural extracellular materials”. Journal of Regenerative Medicine 4(5):747-58
- .Albertson, R.C. and Yelick, P.C. (2009) Methods in Cell Biology, “Morphogenesis of the Jaw: Development Beyond the Embryo,” in Essential Zebrafish Methods - Cell and Developmental Biology. (In Press).
- Lin, Y. and Yelick, P.C. (2007) Translational approaches in tissue engineering and regenerative medicine. Section 7: Methods to Investigate Craniofacial Growth and Development, Chapter 35, Dental and craniofacial tissue engineering, Artech House, Blackwell Publishing (In Press).
- Lin, Y and Yelick, P.C. (2007) Periodontal Applications, Principles of Regenerative Medicine (In Press).
- Yelick, P.C. and Vacanti, J. P. (2006) Bioengineered Teeth from Tooth Bud Cells. Dental Clinics of North America. 50(2):191-203
- Duailibi, S., Duailibi, M., Vacanti, J.P., and Yelick, P.C. (2006). "Prospects for Tooth Regeneration”, Periodontology 2000 41:177-87.
- 20.Yelick, P.C. (2006) “Molecular Biology of Craniofacial Bone Disorders.” Frontiers in Bioscience, (In Preparation).
- .Albertson, R.C. and Yelick, P.C. (2006) “Design and Diversity of the Vertebrate Head” Critical Reviews in Oral Biology and Medicine, (In Preparation).
- Albertson, R.C., Hall, B., and Yelick, P.C. (2005) “Neural Crest” Embryo Today! Issue 2, 2005, May/June publication schedule, (In Preparation).
- Albertson, C. A. and Yelick, P.C. (2004) "The Zebrafish: Cellular and Developmental Biology." “Morphogenesis of the Jaw.” Methods in Cell Biology, Second Edition, Cellular & Developmental Biology and Genetics, Genomics & Informatics, Edited by H. William Detrich, Monte Westerfield, and Leonard I. Zon (pp437-454).
- Yelick, P.C. and Vacanti, J.P. (2004) “Dental Stem Cells,” Handbook of Adult & Fetal Stem Cells, Edited by Irv Weissman, Doug Melton, Catherine Verfaillie, Malcolm Moore, Helen Blau, E. Donnall Thomas, Mike West and Robert Lanza. (In Press).
- Yelick, P.C. (2004) Ten Cate Handbook of Oral History, "Bioengineering Teeth: Dream or Reality?” (In Press.)
- Yelick, P.C. and Schilling, T. (2002). Molecular Dissection of Craniofacial Development in Zebrafish, Critical Reviews in Oral Biology and Medicine 13(4):308-22.
- Yelick, P.C., Stashenko P. Characterization of zebrafish TGF family member receptors. Biological Mechanisms of Tooth Movement and Craniofacial Adaptation (Z. Davidovitch and L.A. Norton, eds.) 1996 pp.423-430.
- Mali, P., Kaipia, A., Kangasniemi, M., Toppari, J., Sandberg, M., Vuorio, E., Yelick, P.C., Hecht, N.B., Parvinen, M. (1990) Expression of nucleoprotein mRNAs during rat spermiogenesis. Nuclear Structure and Function. Ed. J.R. Harris and I.B. Zbarsky, Plenum Press, New York.
- Monteiro, N. and Yelick, P.C. (2015) Chapter 31 Alveolar Complex Regeneration. (In Press) pp.335-341. Published by Wiley Singapore.
- Sanaan, B., Walboomers, F. and Yelick, P.C. (2015) Whole-organ engineering with natural extracellular materials. Chapter X, pp. 1-#. Publisher: Open Book and Monograph Platform, IAPC Publishing, Nanomaterials and Regenerative Medicine. Zagreb, Croatia.
- Human osteoclast-specific and related genes. Patent Number: 5,552,281, Issue Date: August 12, 1997.
- Generation of a Biological Tooth Substitute. Submitted July 2000. Filed, November 2001. Patent Pending.
- "Methods and Compositions for Culturing a Biological Tooth.” Submitted November 2003. U.S. patent application serial no. 09/997,734 has been allowed, and may issue at any time. A continuation application (U.S.S.N.10/859,896) containing additional claims is pending.